We identified both in our genome engineered cells and in three other naturally occurring human melanoma cohorts that samples with low CDC20 express transcriptional programs associated with a neural crest-like or proliferative state, characterized by relatively higher expression of genes like SOX10, which was then validated by siRNA-mediated knockdown of CDC20 in multiple melanoma cell lines (Fig. 5a, Supplemental Data 9, Supplemental Data 10). The gene discussed is CDC20; the disease is melanoma.