Moreover, RNAScope with multiplex IHC staining demonstrates that while immune cells and stromal cells do express SPINK1/Spink1 (Supplementary Fig. S8d-f), only fibroblast cells show an increase in Spink1 expression following chemotherapy treatment in HCC (Supplementary Fig. S8f). This evidence concerns the gene SPINK1 and hepatocellular carcinoma.