HDAC8 and melanoma: As many genes that were upregulated in our RNA-seq and ChIP-Seq analysis are linked to increased invasion, we performed 3D spheroid collagen invasion and Matrigel invasion assays and found that expression of HDAC8 led to increased WM164 and SK-MEL-28 cell invasion when compared to EV cells (Fig. 3a–d).We next asked whether HDAC8 increased the resilience of melanoma cells to shear stress through use of a flow chamber system that mimics the levels of shear experienced in the general circulation25,26.