A Kyoto Encyclopedia of Genes and Genomics (KEGG) pathway analysis revealed that HDAC8 expression increased enrichment for pathways important for melanoma migration including PI3K-AKT signaling, Focal Adhesion, ECM-Receptor Interactions, Regulation of Actin Cytoskeleton, MAPK signaling and RAP1 signaling (Fig. 2b) while decreasing enrichment of some Metabolic Pathways, including glutathione and purine metabolism (Fig. 2c). This evidence concerns the gene AKT1 and melanoma.