The authors selected five cases each of HPV-vaccinated and non-HPV-vaccinated cSCC patients' tumor tissues in order to determine the effect of the HPV vaccine on immune cells in human cSCC tissues, and analyzed the infiltration of CD8, CD4, NK, DC, and Macrophages in cSCC tumors by immunofluorescence. This evidence concerns the gene CD4 and skin squamous cell carcinoma.