To discern the molecular basis of kidney cystogenesis in Tsc1 KO mice, we analyzed and contrasted the RNA transcriptomes in: 1) Tsc1 single KO mice which have numerous cysts and cyst adenomas; and compared it to 2) Tsc1/Foxi1 dKO mice that do not exhibit any kidney cysts. This evidence concerns the gene TSC1 and Complex Cyst of Kidney.