Letrozole, a non-steroidal aromatase inhibitor that blocks the conversion of androgens to estrogens, can induce several reproductive, endocrine, and metabolic abnormalities, including anovulation, ovarian morphology and histoarchitecture changes, insulin resistance, and abnormal hormone levels in female rats, making it a suitable model for studying PCOS pathology and treatment options [22,23]. The gene discussed is INS; the disease is polycystic ovary syndrome.