CFTR and cystic fibrosis: Correction of this mutation was demonstrated previously in a mini-gene assay in HEK293T cells,16 but these cells do not allow for the analysis of functional correction, and so we have now progressed this approach to primary cells from CF donors homozygous for the CFTR 3849+10kb C>T variant, and assessed the efficacy of functional correction in air-liquid interface (ALI) cultures.