In the 3T3-L1 preadipocyte cells, enhanced inhibition of pancreatic lipase was observed when emodin was combined with apigenin and naringenin, further demonstrating that emodin (80 μM) may act as a synergistic agent for the prevention of obesity (Guo et al., 2016). This evidence concerns the gene PNLIP and obesity due to melanocortin 4 receptor deficiency.