Figures 6D, J, R demonstrates that tumors and normal tissue could be visually distinguished by [68Ga]Ga-FAP-2286-ICG FI. The tumor-to-background signal ratio in mice with FaDu, CAL27 and CNE2 tumors at 72 h after injection was favorable, with FaDu tumor-bearing mice having a ratio of 5.95 ± 2.92, CAL27 having a ratio of 2.77 ± 1.70 and CNE2 having a ratio of 5.8 ± 1.15. Notably, a heterogeneous fluorescence signal was observed at the tumor site on both in vivo and in vitro fluorescence images, which aligned with the heterogeneous radioactive uptake observed on PET/CT. Here, FAP is linked to neoplasm.