TARDBP and amyotrophic lateral sclerosis: The cytoplasmic accumulation of exogenous TDP-43, a key feature of ALS neurons (Suk and Rousseaux, 2020), was confirmed by immunocytochemistry, revealing a predominantly nuclear localization of the endogenous TDP-43 in ctr neurons (Neumann et al., 2006) in contrast with an enrichment of both wtTDP-43 and mutTDP-43 in cytoplasmic aggregates (Figure 1C).