TARDBP and proteostasis deficiencies: A neuronal model of TDP-43 proteinopathy was produced by transducing the above UMN cultures with lentiviral particles to deliver tRFP alone (ctr neurons) or a human tRFP-TDP-43 N-ter fusion protein, either wt or carrying the A315T mutation (henceforth defined wtTDP-43 and mutTDP-43 neurons, respectively); constructs are sketched in Figure 1A.