MLKL and brain ischemia: In the MCAO rat model, cerebral ischemia stimulated RIPK1 phosphorylation at the Ser166 residue, RIPK3 phosphorylation at the Ser232 residue and MLKL phosphorylation at the Ser345 residue and significantly increased RIPK1, RIPK3 and MLKL levels, while Nec-1 attenuated these changes by inhibiting phosphorylated RIPK1 kinase [8].