To induce atherosclerosis in AMPKβ1fl/fl or AMPKβ1LysM mice, we injected a gain-of-function PCSK9 AAV via the tail vein to over-express PCSK9 in the liver, which leads to reductions in the liver LDLr and increases in serum cholesterol when mice are fed a Western diet.24 The gene discussed is PCSK9; the disease is atherosclerosis.