According to the ESTIMATE algorithm, B cells, CD4+ T cells, CD8+ T cells, DC cells, macrophages, and neutrophil subsets in tumor tissues of high-risk groups were significantly upregulated significantly higher in the high-risk group (P < 0.05, Fig. 8), indicating that there are more immune cell components in TME in high-risk HCC patients. This evidence concerns the gene CD4 and neoplasm.