By analyzing the differential expression of RNA levels of these nine hub genes in the tumors of LGG patients and paired paracancerous tissues, we also found that compared with the tumor tissues, the expression of paracancerous tissues in LGG patients was significantly increased by 940.93 ± 235.41% at CASP4 (p < 0.001), 76.05 ±  21.42% at PLCG1 (p < 0.001), and 196.50 ± 29.66% at TP63 (p < 0.001) (Figs. 4J–4L). The gene discussed is CASP4; the disease is neoplasm.