Through Illumina methylation 450 (high-throughput methylation chip detection platform) and Spearman’s correlation analysis, it was observed that in RNA sequencing data of colon cancer, TMUB1 expression was negatively correlated with the beta corresponding to the methylation probe CG7847101 (R =  − 0.329, P < 0.001) (Fig. 10B). The gene discussed is TMUB1; the disease is malignant colon neoplasm.