SPP1 and type 2 diabetes mellitus: These results are in line with experimental in vivo data that showed, in T2DM bone healing, a downregulation of expression levels of genes (osteopontin, bone morphogenetic protein‐2, RUNX2, and osteocalcin) known to be associated with MSC proliferation, MSC migration, and osteoblast differentiation.(46, 47, 48, 49, 50, 51) In this study, a 20‐run Plackett–Burman design with 19 parameters was employed, where 16 parameters were real and 3 dummy.