The levels of TNF-α, IL-1β, and NF-κB in the colon tissue homogenate were significantly increased in the colitis group (434.5±53.47 pg/ml, 646.1±58.65 pg/ml and 9.05±0.74 pg/ml, respectively) compared to the healthy control group (139.8±76.57 pg/ml, 142.5±42.09 pg/ml and 1.21±0.75 pg/ml, respectively). This evidence concerns the gene TNF and colitis.