PCOSis a complex, polygenic illness having a significant economicand personal costs.33 Hyperandrogenism,anovulation, and follicular cysts are some of the clinical symptomsof PCOS that are linked to metabolic issues such as insulin sensitivity,hyperinsulinemia, dyslipidemia, and cardiovascular diseases.1 In addition to lowering level of androgens, weightof body and long-term risks for diabetes/cardiovascular disease, PCOS’streatment also seeks to recover ovulation in women who wants to conceive.34 Letrozole, an aromatase (CYP450 enzyme) inhibitor,was employed to induce PCOS. The gene discussed is INS; the disease is diabetes mellitus.