Specifically, the genes in GS/GOGAT pathway were up-regulated in RTN and RTD, and the GDH gene was up-regulated in RTN and RTH, the corresponding product glutamine (Gln) was up-regulated in RTD, whereas the content of glutamate (Glu) in the control group was higher than other N treatment groups. The gene discussed is REN; the disease is renal tubular dysgenesis of genetic origin.