The final selections included elevated CA19-9 (P < 0.001), CA-125 level (P < 0.001), normal CEA level (P < 0.001), tumor size ≥ 5cm (P < 0.001), single lesion (P=0.002), hypo-echogenicity (P < 0.001), irregular shape (P=0.001), tumor necrosis (P < 0.001), and rim APHE (P <0.001) for multivariable logistic regression analysis (Table 3). This evidence concerns the gene CEACAM5 and neoplasm.