Using CRISPR/Cas9-mediated genome editing to disrupt DDX1 gene led to decrease of cell proliferation, suppression of sphere-forming capacity and expression of cancer stem cell marker genes (LGR5, CD133, ALDH1 and SOX2) in CRC cells as well as smaller solid tumor size and lower proliferative marker PCNA expression in xenograft tumor in nude mice (11). Here, DDX1 is linked to colorectal carcinoma.