KLK3 and posterior cortical atrophy: After mTOR ChIP assays, qPCR assays were conducted to examine the expression levels of the mTOR target genes including KLK3, ENO1, SLC26A3 and TMPRSS2. Specifically, KLK3 encodes PSA, ENO1 and SLC26A3 are metabolic genes actively involved in CRPC, and TMPRSS2 is an androgen-driven gene promoting PCa progression/metastasis.