To evaluate the inhibitory effects of miR-99b-5p and Enz on the protein levels and cellular locations of AR/AR-V7, mTOR and SMARCD1, immunofluorescence and western blot assays were performed in the AR-positive lines, including androgen-dependent EA PCa (LNCaP), EA CRPC (22Rv1, C4-2B), and AA PCa (MDA PCa 2b) cells in the absence and/or presence of miR-99b-5p and/or Enz. The gene discussed is SMARCD1; the disease is posterior cortical atrophy.