In this study, we attempted to explore the functional roles of miR-99b-5p in modulating mTOR/AR/SMARCD1 signaling in MDA PCa 2b (an AA PCa cell model with similar features of CRPC C4-2B, that is androgen-independent but responds to Enz treatment) vs. LNCaP (EA PCa, androgen-dependent and Enz-sensitive PCa) and 22Rv1 (androgen-independent and Enz-resistant PCa). This evidence concerns the gene SMARCD1 and posterior cortical atrophy.