MTOR and posterior cortical atrophy: First, immunofluorescence assays were employed to explore the expression levels and subcellular distributions of mTOR, AR, AR-V7, and SMARCD1 in 2D (monolayer) and 3D (organoid) in PCa cell line models derived from EA PCa (LNCaP, PC-3, DU-145, and CRPC lines 22Rv1 and C4-2B) and AA PCa (MDA PCa 2b).