To verify the cellular locations of AR, mTOR, and SMARCD1 under the treatments, western blot analyses were performed using the cytoplasmic and nuclear lysates from EA PCa (LNCaP, 22Rv1 and C4-2B) and AA PCa (MDA PCa 2b) cells grown in the androgen-containing media with the presence/absence of miR-99b-5p and/or Enz. This evidence concerns the gene MTOR and posterior cortical atrophy.