Alternatively, 105 untransfected DCs were cultured with peptide pools (5μg/ml) from VSV, hTERT (melanoma antigen), Cyclin D1 (melanoma antigen), or the peptide SIINFEKL (irrelevant antigen) for 24 hours and then 106 purified CD8+ T cells were added in interferon gamma ELISPOT wells. Here, CD8A is linked to melanoma.