This mutant reintroduces a single copy of γ1 34.5 into the viral genome under the control of the nestin gene enhancer (a glioma-specific enhancer) and the hsp68 promoter.In vitro and in vivo experiments demonstrated robust replication and oncolytic activity of rQNestin34.5 specifically in glioma cells (44). Here, NES is linked to central nervous system cancer.