More interestingly, the metabolic profile was shown to be restricted in a time-dependent manner, as the increase in GLUT-1, HK-2, and PFKFB-3 followed an uprising trend for 48 h in HL-60 and 24 h in THP-1 (the different pattern is probably related to the heterogeneous nature of AML). This evidence concerns the gene PFKFB3 and acute myeloid leukemia.