Using the other two publicly available scRNA-seq datasets, including one mouse sample from the YAP/AKT CCA mouse model and the other mouse from the KRAS/p19 CCA model, we found similar three clusters, with one of them having the highest stemness score and a relatively higher expression of Tm4sf1 (Supplementary Figure S7). This evidence concerns the gene AKT1 and cholangiocarcinoma.