TGFB1 and cancer: Next, to examine the effect of Wnt/β-catenin and TGF-β/BMP pathways on melanoma concerning cancer cell migration and metastasis, we exploited larval zebrafish xenografts by injecting DiO-labeled SK-MEL-28 cells into the yolk sac at 2 dpf, treated them with the agonists of the pathways and observed the cancer cell behavior over time.