Therefore, this project proposes a scientific hypothesis: Through the mathematical modeling of IL-1β/TNF signaling pathway screened by high-throughput sequencing, the regulatory effect of signal molecules in the IL-1β/TNF signaling pathway on chondrocyte apoptosis can be systematically analyzed, and new targets for the treatment of osteoarthritis can be further explored through the sensitivity analysis of parameters. This evidence concerns the gene IL1B and osteoarthritis.