Granting the wide debate over astrocyte role as HIV reservoir due to insufficient CD4 expression and the inefficient in vitro infection (Woodburn et al., 2022; Li et al., 2020; Edara et al., 2020), once the barrier of viral entry is overcome, stable provirus integration changes astrocyte phenotype and a productive infection is established based on viral promoter (Malik et al., 2021; Borrajo et al., 2021a; Edara et al., 2020). This evidence concerns the gene CD4 and infection.