According to the ROC analysis of our study, when only the top four genes with the highest area under the curve (AUC) were included, higher expressions of ATP5C1 (AUC = 0.682; p < 0.001), RAC3 (AUC = 0.677; p < 0.001), ADAM22 (AUC = 0.643; p < 0.001), and SHANK1 (AUC = 0.605; p = 0.007), and lower expressions of C1RL (AUC = 0.711; p < 0.001), CHL1 (AUC = 0.701; p < 0.001), EFEMP2 (AUC = 0.699; p < 0.001), and PGCP (AUC = 0.695; p < 0.001) in GBM tissues were associated with the long-term survival (more than 2.5 years) in patients with GBM (Fig 3E). This evidence concerns the gene EFEMP2 and glioblastoma.