Additionally, we observed the expression of these significant signature genes and found that GREB1, CDK4, and GPR125 showed highly consistent expression in Cluster 3 of the three NB cohorts, with CDK4 and GPR125 predicting an AUC of greater than 0.7 for Cluster 1&2 and Cluster 3 in all three NB cohorts, implying good discriminatory ability (Figure 4F, 4G). The gene discussed is ADGRA3; the disease is neuroblastoma.