To investigate the inflammatory regulation in DSS-induced colitis, the mRNA expression of pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6), iNOS, and cyclooxygenase-2 (COX-2) in the colon tissue of DSS-induced C57BL/6 mice was determined using RT-qPCR. The gene discussed is PTGS2; the disease is colitis.