The results showed a significant association (P < 0.05) between NASH- and obesity-related parameters and the gut microbiota of GAN-fed mice, including body weight, total fat, TC, LDL, AST, liver weight, NAS, steatosis, lobular inflammation, hepatocyte ballooning, TNF-ɑ, IL-1β, and IL-6. The gene discussed is TNF; the disease is steatosis.