To investigate the effect of BFO on the inflammatory responses of DSS‐induced colitis mice, we determined the mRNA expression levels colon‐related genes, including IL‐1β, IL‐6, TNF‐α, inducible nitric oxide synthase (iNOS), and cyclooxygenase 2 (COX‐2), by qPCR (Figure 6). This evidence concerns the gene TNF and colitis.