WB was used to detect NF-κBp65 and p-NF-κBp65 expression.<h4>Results</h4>Compared with the AM group, the ISL pretreatment promoted cell proliferation and migration, inhibited cell apoptosis, increased the total length and total branches of angiogenesis, and downregulated p-NF-κBp65 expression.<h4>Conclusion</h4>ISL shows promise in the prevention and treatment of clinical phlebitis and can be used as a potential therapeutic drug to prevent phlebitis. The gene discussed is NFASC; the disease is phlebitis.