Furthermore, we revealed that the development of CFA chronic pain‐induced anxiety‐like behaviors is attributed to the dysfunction of rACC CaMKII neurons projecting to DRN serotonergic neurons (rACCCaMKII‐DRN5‐HT) but not rACC CaMKII neurons projecting to DRN GABAergic neurons (rACCCaMKII‐DRNGABA). Here, CAMK2G is linked to Anxiety.