Images were acquired at 0 and 48 h in cell scratch assay, and the results showed that PSA-dCas9-KRAB also reduced the relative migration rates of several transfected prostate cancer cell lines compared with that transfected with the the non-target gRNA and dCas9-KRAB expression control (Fig. 3B). This evidence concerns the gene KLK3 and Familial prostate cancer.