We tested the effect of ibrutinib on cell proliferation by incubating primary CLL and RT samples, and cells from PDXs for 6 days in an enriched medium containing ODN2006 + IL-15 (EM), which was the best condition culture to maintain viability and to induce proliferation on cells (Fig. S8). This evidence concerns the gene IL15 and B-cell chronic lymphocytic leukemia.