Most AML-associated mutations and chromosome rearrangements resulted in splicing impacts, most significantly associated with splicing-factor mutations (SRSF2-P95*, U2AF1-S34*, SF3B1, SF3A1, U2AF1-Q157*, SF1), common mutations (NPM1, TP53), CN-AML, complex karyotype, and chromosome rearrangements resulting in gene fusions involving CBFB and KMT2A (Fig. 4A, B). Here, NPM1 is linked to cyclic hematopoiesis.