The differential activation of immunosuppressive pathways such as angiogenesis, epithelial mesenchymal transition, TGF-beta signaling along with a loss in activity of anti-tumor immunity pathways such as NOS1 signature, MAPK up genes and telomerase extension by telomerase were multiple contributing factors for driving the difference between PANoptosis High and Low cluster, specifically in LGG. The gene discussed is NOS1; the disease is neoplasm.