In contrast to what we observed in mice with CerS6 deficiency in Nkx2.1-expressing cells, inhibiting CerS1 in neurons of the hypothalamus did not affect HFD-induced obesity, systemic insulin sensitivity, or glucose tolerance (Supplementary Fig. 4d-i). This evidence concerns the gene NKX2-1 and obesity due to melanocortin 4 receptor deficiency.