State anxiety was negatively associated with the percentage of FoxP3+ Tregs in the parent gate (β = −2.88, p = 0.031) as well as subpopulations of Helios+ (β = −3.29, p = 0.019) and TIM-3+ (β = −3.17, p = 0.008) Treg cells at 12 weeks. Here, FOXP3 is linked to anxiety disorder.