Further treatment with anti-HMGB1 antibodies decreased tumor size, ERK1/2 activation, and PCNA expression in azoxymethane/dextran sodium sulfate-treated WT mice, implying that GSDME-associated pyroptosis accelerates colitis-associated colorectal cancer progression via intracellular HMGB1 release, triggering cancer cell proliferation (95). This evidence concerns the gene HMGB1 and colorectal cancer.