discovered that accessorial caspase-1 expression, activation of GSDMD, excessive IL-1β release, translocation of HMGB1 to the cytoplasm, membrane hole formation, and other pyroptosis characteristics were observed in breast cancer cells treated with DHA when compared with the untreated group, implying that DHA may be an inducer of pyroptosis in breast cancer cells (78). This evidence concerns the gene HMGB1 and breast cancer.