Using criteria log2 fold-change (log2FC) > 1 and P < 0.05, we performed serial Venn analysis and identified 3 genes (protein kinase AMP-activated catalytic subunit alpha 2 [Prkaa2], Mmp9, and Gdf3) with significantly differential expression in Znfx1–/– BMDMs compared with in WT BMDMs at both 6 and 24 hpi, regardless of H37Rv infection (Supplemental Table 3). This evidence concerns the gene GDF3 and infection.