Furthermore, expression of Akt overexpression in the fat body of tumour animals (QRasV12, scribRNAi), caused an upregulation of pAkt (Fig 2A–C), but an unexpected downregulation of the TGF‐β signalling readout pMad (Fig 2D–F), indicating that an upregulation of insulin/PI3K signalling maybe correlated with a downregulation of TGF‐β signalling. The gene discussed is INS; the disease is neoplasm.