SPN and neoplasm: Increased tumor infiltration by monocytes, classified by high Ly6c1 expression, was observed across all treatment groups as compared to sham control mice (Fig. 5i), with an enrichment of non-classical monocytes characterized by Spn, Cx3cr1, and Tnfrsf1b expression32 in groups receiving anti-PD-1 treatment (Fig. 5j).