Herein, we tested the cytotoxicity of the differentiated CAR-TIM3 NK-like cells against TIM3-positive AML cells, including CD34+ KG-1 cells and CD34− U937 cells, which were introduced with TIM3 transgene, hereafter called TIM3-overexpressing U937 cells (Additional file 1: Fig. S2). This evidence concerns the gene CD34 and acute myeloid leukemia.