The flow cytometric definitions of each cell fraction were as follows: lung cancer cell (GFP + /CD45−), tumor-associated macrophages (TAM; GFP-/CD45 + /CD206 +), cancer-associated myofibroblasts (CAMF; GFP-/CD45-/CD31-/CD326-/CD140a + /αSMA +), regulatory T cells (Treg; GFP−/CD3 + /CD4 + /CD25 +), and cytotoxic T lymphocytes (CTL; GFP-/CD3 + /CD8 +) (Fig. 5A). This evidence concerns the gene ACTA1 and lung cancer.