Second, to further explore the influence of the adaptive immune system of NOD and 116C-NOD models on their faecal microbiota, the gut microbiota and intestinal wall integrity of the immunodeficient variants NOD.RAG-2−/− and 116C-NOD.RAG-2−/−, as well as a non-T1D-prone mouse control, were also studied. Here, RAG2 is linked to type 1 diabetes mellitus.