To explore whether miR-dDiAs results in DG-related circuit disorders through RTN3 upregulation in isolated AD mice, we injected adeno-associated virus (AAV)-packaged miR decoy for miR-218 and miR-124 (AAV2/8-miR-dDiAs decoy, 1 × 1012 IU/μL, 1 μL) or mixed viruses of AAV-packaged decoy and AAV-packaged short hairpin RNA (shRNA) that specifically targets Rtn3 (AAV2/8-miR-dDiAs decoy, 1 × 1012 IU/μL, 1 μL mixed with AAV2/8-Rtn3 shRNA, 1 × 1012 IU/μL, 1 μL) or the control virus into the DG of group-housed c57 mice at 2 months (Fig. S7A–E). The gene discussed is RTN3; the disease is Alzheimer disease.